Coding
cotZ

Part:BBa_K823031:Design

Designed by: Franziska Dürr   Group: iGEM12_LMU-Munich   (2012-09-06)

cotZ: B. subtilis spore crust protein (-2aa)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 42
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed it in Freiburg standard (Assembly 25) and used it for the display of fusion proteins on B. subtilis spores.


Source

B. subtillis W168 genome

References

[http://www.ncbi.nlm.nih.gov/pubmed/21665972 Imamura et la., 2011]